Prion infectivity in variant Creutzfeldt-Jakob disease rectum.
Gut
; 56(1): 90-4, 2007 Jan.
Article
em En
| MEDLINE
| ID: mdl-16763054
ABSTRACT
BACKGROUND:
Disease-related prion protein (PrP(Sc)) is readily detectable in lymphoreticular tissues in variant Creutzfeldt-Jakob disease (vCJD), but not in other forms of human prion disease. This distinctive pathogenesis, with the unknown population prevalence of asymptomatic vCJD infection, has led to significant concerns that secondary transmission of vCJD prions will occur through a wide range of surgical procedures. To date PrP(Sc)prion infectivity ratios have not been determined in vCJD, and it is unknown whether vCJD prions are similar to experimental rodent prions, where PrP(Sc) concentration typically reflects infectious prion titre.AIM:
To investigate prion infectivity in vCJD tissue containing barely detectable levels of PrP(Sc).METHODS:
Transgenic mice expressing only human PrP (Tg(HuPrP129M(+/+)Prnp(o/o))-35 and Tg(HuPrP129M(+/+)Prnp(o/o))-45 mice) were inoculated with brain or rectal tissue from a previously characterised patient with vCJD. These tissues contain the maximum and minimum levels of detectable PrP(Sc) that have been observed in vCJD.RESULTS:
Efficient transmission of prion infection was observed in transgenic mice inoculated with vCJD rectal tissue containing PrP(Sc) at a concentration of 10(4.7)-fold lower than that in vCJD brain.CONCLUSIONS:
These data confirm the potential risks for secondary transmission of vCJD prions via gastrointestinal procedures and support the use of PrP(Sc) as a quantitative marker of prion infectivity in vCJD tissues.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Reto
/
Encéfalo
/
Príons
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Síndrome de Creutzfeldt-Jakob
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
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Humans
Idioma:
En
Revista:
Gut
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Reino Unido