Novel dermaseptins from Phyllomedusa hypochondrialis (Amphibia).
Biochem Biophys Res Commun
; 347(3): 739-46, 2006 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-16844081
ABSTRACT
Six new antimicrobial peptides structurally related to the dermaseptin family have been isolated from the skin secretion of the amphibian Phyllomedusa hypochondrialis. The primary structures of these molecules named as DShypo 01, 02, 03, 04, 06, and 07 were determined by de novo MS/MS experiments, Edman degradation, and cDNA sequencing. The fifth peptide was found to be precisely the same DS 01 from Phyllomedusa oreades previously described by our group. The majority of the peptides purified from the crude skin secretion could be directly localized and mapped onto a freshly dissected dorsal skin fragment using mass spectrometry-imaging techniques. Comparisons between peptides and commercial drugs on their antibacterial and anti-Leishmania amazonensis efficiencies, associated with peptide lytic effects on mammalian blood cells and surface plasmon resonance interaction studies on immobilized DMPC vesicles, were also performed.
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Base de dados:
MEDLINE
Assunto principal:
Anuros
/
Peptídeos Catiônicos Antimicrobianos
/
Proteínas de Anfíbios
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Brasil