Effects of etamsylate on equine platelets: in vitro and in vivo studies.
Vet J
; 174(2): 325-9, 2007 Sep.
Article
em En
| MEDLINE
| ID: mdl-16904352
ABSTRACT
The aim of this study was to investigate whether etamsylate produces equine platelet activation. In vitro and in vivo studies were designed in which seven and eight adult healthy horses were included, respectively. In the in vitro study, citrated blood was incubated with different concentrations of etamsylate, and P-selectin expression and annexin V binding were determined by flow cytometry. In the in vivo study, blood was collected before and 1 and 2h after IV administration of etamsylate, and P-selectin expression was evaluated. In the in vitro study, a significant increase in P-selectin expression, leukocyte-platelet aggregate formation and annexin V binding were observed. In the in vivo study, a marked increase in P-selectin expression and heterotypic aggregate formation was seen in two and five horses, respectively, although no significant differences were detected when analyzing results from all the animals together. The results of the in vitro study indicate that etamsylate produces a pre-activation state in equine platelets, but this fact could be confirmed by the in vivo study.
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Base de dados:
MEDLINE
Assunto principal:
Plaquetas
/
Hemostáticos
/
Ativação Plaquetária
/
Etamsilato
/
Cavalos
Limite:
Animals
Idioma:
En
Revista:
Vet J
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Espanha