Isolation and characterization of full-length recombinant cattle PrPC protein.

Kal'nov, S L; Grigor'ev, V B; Alekseev, K P; Vlasova, A N; Gibadulin, R A; Pokidyshev, A N; Balandina, M V; Tsibezov, V V; Verkhovskii, O A

Kal'nov, S L; Grigor'ev, V B; Alekseev, K P; Vlasova, A N; Gibadulin, R A; Pokidyshev, A N; Balandina, M V; Tsibezov, V V; Verkhovskii, O A.

Afiliação

Kal'nov SL; D I Ivanovsky Institute of Virology, Russian Academy of Medical Sciences. kalnov@narvac.com

Bull Exp Biol Med ; 141(1): 62-5, 2006 Jan.

Article em En | MEDLINE | ID: mdl-16929966

ABSTRACT

ABSTRACT

Full-length Bos taurus PrPC protein was obtained in the eu- and prokaryotic expression systems. Immunoblotting and indirect enzyme immunoassay demonstrated high specificity and antigenic activity of full-length proteins in the reactions with monoclonal antibodies (anti-SAF-32 and VRQ-84). Membrane location of recombinant PrPC protein in insect cells was shown by immunofluorescent analysis.

Assuntos

Proteínas PrPC/biossíntese; Proteínas Recombinantes/biossíntese; Animais; Anticorpos Monoclonais/imunologia; Antígenos/análise; Baculoviridae/genética; Bovinos; Membrana Celular/química; Proteínas PrPC/análise; Proteínas PrPC/imunologia; Proteínas Recombinantes/análise; Proteínas Recombinantes/imunologia

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Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes / Proteínas PrPC Limite: Animals Idioma: En Revista: Bull Exp Biol Med Ano de publicação: 2006 Tipo de documento: Article

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