[Quercetin inhibits growth and induces apoptosis of human gastric carcinoma cells].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 22(5): 585-7, 2006 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-16948901
ABSTRACT
AIM:
To study the effect of quercetin on the growth and apoptosis of human gastric carcinoma cell line MGC-803.METHODS:
The measurement of inhibitory rate and apoptotic index(AI) of quercetin were done by MTT assay and TUNEL assay. The positive expression rate of P53, C-myc and P16 were detected by immunocytochemical staining.RESULTS:
Quercetin at concentrations ranging from 40 mumol/L to 100 mumol/L significantly inhibited the proliferation of MGC-803 cells in a dose- and time-dependent manner (P<0.01). TUNEL assay indicated that the number of apoptotic cells in quercetin-treated group was greater than that in the control group (P<0.01). Expression of P53 and C-myc protein decreased following quercetin induction in a dose-dependent manner, whereas P16 expression increased significantly compared with that of the control group (P<0.01).CONCLUSION:
Quercetin can inhibit the growth and induce apoptosis of MGC-803 cells in a dose- and time-dependent manner. Its mechanisms may be relevant to the down-regulation of P53 and C-myc protein expression as well as up-regulation of P16 expression.
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Base de dados:
MEDLINE
Assunto principal:
Quercetina
/
Neoplasias Gástricas
/
Apoptose
Limite:
Humans
Idioma:
Zh
Revista:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
China