A simple DNA stretching method for fluorescence imaging of single DNA molecules.
Nucleic Acids Res
; 34(17): e113, 2006.
Article
em En
| MEDLINE
| ID: mdl-16971459
ABSTRACT
Stretching or aligning DNA molecules onto a surface by means of molecular combing techniques is one of the critical steps in single DNA molecule analysis. However, many of the current studies have focused on lambda-DNA, or other large DNA molecules. There are very few studies on stretching methodologies for DNA molecules generated via PCR (typically smaller than 20 kb). Here we describe a simple method of stretching DNA molecules up to 18 kb in size on a modified glass surface. The very low background fluorescence allows efficient detection of single fluorescent dye labels incorporated into the stretched DNA molecules.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Microscopia de Fluorescência
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos