[Experimental study on ultrasound heating affecting the sensitivity of oral carcinoma to chemotherapy drugs].

ABSTRACT

OBJECTIVE: To explore the influence of chemosensitivity of Tca8113 cells by modified MTT assay after the animal model of Tca8113 were treated by the ultrasound hyperthermia. METHODS: The MTT assay of the BALB/C nu/nu mice model of Tca8113 cells treated by the ultrasound hyperthermia in vivo was performed. RESULTS: The chemosensitivity to the 9 kinds of drugs demonstrated no significant differences between the Tca8113 cells in the control group, the 39 degrees C-treated group and the groups treated from 41 degrees C to 44 degrees C. But significant differences between the 40 degrees C-treated group and the 41 degrees C or 42 degrees C-treated group existed. In the heating-time grades test, there were no significant differences in the chemosensitivity to the 9 kinds of drugs between these three pairs of group (the control group and the 15 min-treated group, the 30 min-treated and the 45 min-treated group, the 60 min-treated and the 75 min-treated group). But there were significant differences between the 30 min-treated or the 45 min-treated group and the 60 min-treated or the 75 min-treated group. CONCLUSION: Ultrasound hyperthermia performed in 42 degrees C for 30-45 min can improve the chemosensitivity of Tca8113 cells to some drugs significantly, which confirms the rationality of synchronous combination of hyperthermia and chemotherapy in the chemosensitivity point of view for the first time.