Peroxisome targeting signal 1: is it really a simple tripeptide?
Biochim Biophys Acta
; 1763(12): 1565-73, 2006 Dec.
Article
em En
| MEDLINE
| ID: mdl-17007944
ABSTRACT
Originally, the peroxisomal targeting signal 1 (PTS1) was defined as a tripeptide at the C-terminus of proteins prone to be imported into the peroxisomal matrix. The corresponding receptor PEX5 initiates the translocation of proteins by identifying potential substrates via their C-termini and trapping PTS1s through remodeling of its TPR domain. Thorough studies on the interaction between PEX5 and PTS1 as well as sequence-analytic tools revealed the influence of amino acid residues further upstream of the ultimate tripeptide. Altogether, PTS1s should be defined as dodecamer sequences at the C-terminal ends of proteins. These sequences accommodate physical contacts with both the surface and the binding cavity of PEX5 and ensure accessibility of the extreme C-terminus. Knowledge-based approaches in applied Bioinformatics provide reliable tools to accurately predict the peroxisomal location of proteins not yet determined experimentally.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Sinais Direcionadores de Proteínas
/
Receptores Citoplasmáticos e Nucleares
/
Peroxissomos
/
Transporte Proteico
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Áustria