Response surface methodology in the development of a stacking-sensitive capillary electrophoresis method by field-amplified injection for the analysis of tricyclic antidepressants in the presence of salts.
J Sep Sci
; 29(13): 2091-7, 2006 Aug.
Article
em En
| MEDLINE
| ID: mdl-17017024
The work presented here explores the possibilities of the electrokinetic injection (EK) to achieve sensitive methods for the determination of tricyclic antidepressants in biological samples (serum). The addition of ACN to the sample, with high content in salts, causes stacking at the tip of the capillary, in a similar way as for hydrodynamic injection. An experimental design with the response surface methodology has been used to find the optimum composition of the matrix of the sample (sodium chloride and ACN percentages) and the conditions for the EK (water-plug length, time, and voltage of injection) in few experiments. The composition of the separation buffer was the same as utilized in a previous paper. The use of a bubble capillary to reach lower detection limits implies a loss of the resolution and requires a new optimization. Finally, a comparison between electrokinetic and hydrodynamic injections is made.
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Base de dados:
MEDLINE
Assunto principal:
Eletroforese Capilar
/
Antidepressivos Tricíclicos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Sep Sci
Ano de publicação:
2006
Tipo de documento:
Article