Jug r 4, a legumin group food allergen from walnut (Juglans regia Cv. Chandler).
J Agric Food Chem
; 54(21): 8369-75, 2006 Oct 18.
Article
em En
| MEDLINE
| ID: mdl-17032053
ABSTRACT
Allergy to walnut is the most frequently reported tree nut allergy in the United States. Walnut 2S albumin, a vicilin-like protein, and a lipid transfer protein allergen have previously been described. Our objective was to clone and express a cDNA encoding a legumin group protein, assess IgE-binding with sera from walnut allergic patients, and investigate cross-reactivity with selected nuts. Primers were used to obtain the cDNA by 5' and 3' rapid amplification of cDNA ends from walnut mRNA. The cDNA was subcloned into the pMAL-c2X vector and the recombinant fusion protein, named rJug r 4, was expressed in Escherichia coli. The obtained cDNA encoded a precursor protein with a predicted molecular weight of 58.1 kD, which showed significant sequence homology to hazelnut and cashew legumin allergens. Serum IgE from 21 of 37 (57%) patients bound the rJug r 4 fusion protein. In vitro cross-reactivity was demonstrated with hazelnut, cashew, and peanut protein extracts.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Alérgenos
/
Juglans
/
Antígenos de Plantas
Limite:
Humans
Idioma:
En
Revista:
J Agric Food Chem
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos