[Study on vitrification of porcine embryos by open pulled straw method].
Sheng Wu Gong Cheng Xue Bao
; 22(5): 845-9, 2006 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-17037213
ABSTRACT
291 embryos (Blastocyst/Morula) from 20 donor sows were vitrified by two step method with OPS (open pulled straw) in solution I (TCM199 + 20% FBS + 10% EG + 10% DMSO) for 3min, and solution II (TCM199 + 20% FBS + 20% EG + 20% DMSO + 0.4mol/L SUC) for 1min, stored in liquid nitrogen for 3 months, and transferred into 8 recipient sows after warming, one recipient sow was pregnant and 8 alive piglets were born. This is the first paper to report getting alive piglets by vitrification in China.
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Base de dados:
MEDLINE
Assunto principal:
Suínos
/
Blastocisto
/
Criopreservação
Limite:
Animals
/
Pregnancy
Idioma:
Zh
Revista:
Sheng Wu Gong Cheng Xue Bao
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
China