Disruption of the salivary protein 14 in Ixodes scapularis nymphs and impact on pathogen acquisition.
Am J Trop Med Hyg
; 75(4): 677-82, 2006 Oct.
Article
em En
| MEDLINE
| ID: mdl-17038693
ABSTRACT
We previously examined the physiological role of the anticoagulant salivary protein 14 (salp14) in adult Ixodes scapularis and showed that Salp14 played a role in tick feeding and engorgement. We now analyze whether the disruption of the salp14 family expression by RNA interference affects tick weight in naïve nymph I. scapularis. Salp14 expression after dsRNA injection was significantly reduced, as shown by mRNA and protein analysis. However, nymph engorgement weight was not altered in salp9pac (salp14 paralog) dsRNA-injected ticks. We also determined Borrelia burgdorferi and Anaplasma phagocytophilum acquisition in I. scapularis nymphs that had reduced Salp14 expression. B. burgdorferi and A. phagocytophilum acquisition was not affected 72 hours after feeding. Our results suggest that different mechanisms govern nymph and adult feeding in I. scapularis.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Proteínas e Peptídeos Salivares
/
Ixodes
/
Borrelia burgdorferi
/
Anaplasma phagocytophilum
/
Interferência de RNA
Limite:
Animals
Idioma:
En
Revista:
Am J Trop Med Hyg
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos