Quantitative analysis of complex peptide mixtures using FTMS and differential mass spectrometry.
J Am Soc Mass Spectrom
; 18(2): 226-33, 2007 Feb.
Article
em En
| MEDLINE
| ID: mdl-17070068
ABSTRACT
Label-free LC-MS profiling is a powerful quantitative proteomic method to study relative peptide abundances between two or more biological samples. Here we demonstrate the use of a previously described comparative LC-MS method, differential mass spectrometry (dMS), to analyze high-resolution Fourier transform mass spectrometry (FTMS) data for detection and quantification of known peptide differences between two sets of complex mixtures. Six standard peptides were spiked into a processed plasma background at fixed ratios from 1.251 to 41 to make two sets of samples. The resulting mixtures were analyzed by microcapillary LC-FTMS and dMS. dMS successfully identified five out of the six peptides as statistically significant differences (p
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Proteínas
/
Espectroscopia de Infravermelho com Transformada de Fourier
/
Misturas Complexas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Am Soc Mass Spectrom
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos