Rapid and low-cost colorimetric method using 2,3,5-triphenyltetrazolium chloride for detection of multidrug-resistant Mycobacterium tuberculosis.
J Med Microbiol
; 55(Pt 12): 1657-1659, 2006 Dec.
Article
em En
| MEDLINE
| ID: mdl-17108268
ABSTRACT
A rapid and inexpensive method for the detection of drug resistance in Mycobacterium tuberculosis is essential for the effective control of tuberculosis. The aim of this study was to evaluate a colorimetric method using 2,3,5-triphenyltetrazolium chloride (TTC) for antibiotic susceptibility testing of M. tuberculosis isolates. Eleven multidrug-resistant (MDR) isolates of M. tuberculosis and 12 isolates which were susceptible to rifampicin (RIF) and isoniazid (INH) were used. The test was performed with a critical concentration of 0.2 microg ml(-1) for INH and 2.0 microg ml(-1) for RIF in 7H9GC broth with 0.625 microg TTC ml(-1). Each isolate was inoculated under these conditions and inspected daily for colour changes; the results were obtained after a mean of 4.9 days. The sensitivity and specificity of this method were 100 % and 92 %, respectively, for both antibiotics. Considering the speed, technical ease and cost-effectiveness of this method, the TTC assay is a good alternative method for drug susceptibility testing of M. tuberculosis isolates.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Rifampina
/
Tuberculose
/
Testes de Sensibilidade Microbiana
/
Técnicas Bacteriológicas
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Farmacorresistência Bacteriana Múltipla
/
Isoniazida
/
Antibióticos Antituberculose
/
Mycobacterium tuberculosis
Tipo de estudo:
Diagnostic_studies
/
Health_economic_evaluation
Limite:
Humans
Idioma:
En
Revista:
J Med Microbiol
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Irã