Pol I transcription and pre-rRNA processing are coordinated in a transcription-dependent manner in mammalian cells.
Mol Biol Cell
; 18(2): 394-403, 2007 Feb.
Article
em En
| MEDLINE
| ID: mdl-17108330
ABSTRACT
Pre-rRNA synthesis and processing are key steps in ribosome biogenesis. Although recent evidence in yeast suggests that these two processes are coupled, the nature of their association is unclear. In this report, we analyze the coordination between rDNA transcription and pre-rRNA processing in mammalian cells. We found that pol I transcription factor UBF interacts with pre-rRNA processing factors as analyzed by immunoprecipitations, and the association depends on active rRNA synthesis. In addition, injections of plasmids containing the human rDNA promoter and varying lengths of 18S rDNA into HeLa nuclei show that pol I transcription machinery can be recruited to rDNA promoters regardless of the product that is transcribed, whereas subgroups of pre-rRNA processing factors are recruited to plasmids only when specific pre-rRNA fragments are produced. Our observations suggest a model for sequential recruitment of pol I transcription factors and pre-rRNA processing factors to elongating pre-rRNA on an as-needed basis rather than corecruitment to sites of active transcription.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
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DNA Ribossômico
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RNA Ribossômico
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Precursores de RNA
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Processamento Pós-Transcricional do RNA
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Proteínas Pol1 do Complexo de Iniciação de Transcrição
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Mol Biol Cell
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos