Estimating true antimalarial efficacy by heteroduplex tracking assay in patients with complex Plasmodium falciparum infections.
Antimicrob Agents Chemother
; 51(2): 521-7, 2007 Feb.
Article
em En
| MEDLINE
| ID: mdl-17116685
ABSTRACT
Heteroduplex tracking assays (HTAs) of Plasmodium falciparum merozoite surface protein 1 block-2 were used to assess complexity of infection and treatment efficacy in a trial of three antimalarial treatments in 141 Malawian pregnant women. An elevated complexity of infection (COI) was associated with anemia, parasite burden, and human immunodeficiency virus infection but was not associated with age or gravidity. Comparisons of HTA patterns before and after treatment allowed the classification of 20 of 30 (66%) recurrent episodes as either definite treatment failures or reinfections. An elevated COI was strongly associated with treatment failure (P=0.003). An algorithm was developed to assign a probability of failure for the 10 indeterminate participants, some of whose infections shared a single variant of high prevalence (>10%). By summing these probabilities, treatment efficacy was estimated.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plasmodium falciparum
/
Bioensaio
/
Malária Falciparum
/
Antimaláricos
Tipo de estudo:
Clinical_trials
/
Diagnostic_studies
/
Health_technology_assessment
/
Risk_factors_studies
Limite:
Animals
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Antimicrob Agents Chemother
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos