FcRgamma chain does not replace CD3zeta chain in CD3zeta-deficient T lymphocytes of patients with gastric adenocarcinoma.
Mol Immunol
; 44(9): 2400-5, 2007 Mar.
Article
em En
| MEDLINE
| ID: mdl-17134755
ABSTRACT
Defective CD3zeta chain expression has been reported in T lymphocytes of patients with inflammatory diseases, such as systemic lupus erythematosus or osteoarthritis, and with cancer. In lupus, the absent CD3zeta chain is replaced by the FcRgamma chain, rendering the T cells hyper responsive. However, there are no data on T lymphocytes from patients with cancer. In this study, the presence of the FcRgamma chain and its associated kinase, Syk, was analysed in patients with gastric adenocarcinoma and healthy subjects. Western blot and immunoprecipitation experiments were carried out with total cell or lipid raft extracts from fresh peripheral blood mononuclear cells or T lymphocytes, and Herpesvirus saimiri-derived T-cell lines (of blood or tissue origin). Our results revealed that the absent CD3zeta chain in cancer T lymphocytes was not replaced by FcRgamma either in fresh T cells or T-cell lines, in contrast to lupus T cells. This altered expression of signalling molecules in T lymphocytes of cancer patients, would explain their low proliferative capacity. Our T-cell lines represent tools to unveil the signalling abnormalities of cancer T lymphocytes.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Gástricas
/
Receptores Fc
/
Linfócitos T
/
Adenocarcinoma
/
Complexo CD3
Limite:
Humans
Idioma:
En
Revista:
Mol Immunol
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Espanha