Modification of the pig CFTR gene mediated by small fragment homologous replacement.
Ann N Y Acad Sci
; 1082: 120-3, 2006 Oct.
Article
em En
| MEDLINE
| ID: mdl-17145933
ABSTRACT
The generation of a pig model of cystic fibrosis (CF) is a multistep process. Initial steps in this process involved the design and cloning of a small DNA fragment (SDF) or large oligodeoxynucleotide (LODN) that contains the F508del mutation and a silent restriction fragment length polymorphism causing mutation. This SDF/LODN was transfected into wild-type (WT) pig fetal fibroblast with the intention of modifying the pig genomic DNA by small fragment homologous replacement (SFHR). The targeted deletion (F508del) was detected in a subpopulation of transfected cells by allele-specific polymerase chain reaction (AS-PCR).
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Base de dados:
MEDLINE
Assunto principal:
Marcação de Genes
/
Regulador de Condutância Transmembrana em Fibrose Cística
/
Fibrose Cística
/
Modelos Animais de Doenças
Limite:
Animals
Idioma:
En
Revista:
Ann N Y Acad Sci
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos