Development of CD25- regulatory T cells following heart transplantation: evidence for transfer of long-term survival.
Eur J Immunol
; 37(1): 147-56, 2007 Jan.
Article
em En
| MEDLINE
| ID: mdl-17171754
ABSTRACT
Donor-specific heart allograft acceptance can be induced in the MHC-mismatched LEW.1 W to LEW.1A rat by donor-specific transfusions. Whereas the induction phase of tolerance has been studied in detail, its maintenance remained poorly understood. Here, we performed a side-by-side comparison of CD25+ and CD25- splenic T cells of 100-day tolerant rats. Administration of CD25- T cells from tolerant rats to sublethally irradiated recipients transferred long-term graft survival. These CD25- T cells displayed a decreased donor-specific response in the mixed lymphocyte reaction and presented suppressive activity. These CD25- T cells accumulated IFN-gamma, IL-10 and Foxp3 transcripts. The in vitro suppressive activity of CD25- T cells required both cell contact and soluble factors (IL-10 and IFN-gamma). The CD25+ T cells from tolerant rats did not show any modification of their regulatory properties. We show that splenic CD25- T cells of tolerant rats contribute to the maintenance of tolerance following the transplantation. Our data show that regulatory T cells are not restricted to the CD4+ CD25+ T cell subset and provide new insights on the mechanisms of tolerance to allograft following donor cell priming.
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Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
/
Transplante de Coração
/
Linfócitos T Reguladores
/
Transferência Adotiva
/
Subunidade alfa de Receptor de Interleucina-2
/
Sobrevivência de Enxerto
Limite:
Animals
Idioma:
En
Revista:
Eur J Immunol
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
França