Non-specific oligodendrocyte cytotoxicity mediated by soluble products of activated T cell lines.
J Neuroimmunol
; 35(1-3): 261-71, 1991 Dec.
Article
em En
| MEDLINE
| ID: mdl-1720136
ABSTRACT
Supernates from myelin basic protein (MBP)-reactive T cell lines have been tested by a battery of assays for cytotoxicity against oligodendrocytes in vitro. All supernates tested demonstrated cytotoxic activity in both a dose- and time-dependent manner that ranged from 29.1% to 55.8% after 48 h incubation. Oligodendrocyte cytotoxicity mediated by MBP-reactive T cell lines was not antigen specific since lines reactive with purified protein derivative (PPD) of tuberculin showed similar cytotoxicity. Supernates cytotoxic to oligodendrocytes were equally effective against syngeneic and non-syngeneic target cells, but had no effect upon astrocytes. Neutralization studies revealed that oligodendrocyte cytotoxicity mediated by MBP-reactive T cell supernates could only be partially attributed to lymphotoxin/tumor necrosis factor activity, and was not associated with perforin, serine proteinase or N-methyl-D-aspartate (NMDA) receptor agonists.
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Base de dados:
MEDLINE
Assunto principal:
Ativação Linfocitária
/
Linfócitos T
/
Oligodendroglia
/
Citotoxicidade Imunológica
Limite:
Animals
Idioma:
En
Revista:
J Neuroimmunol
Ano de publicação:
1991
Tipo de documento:
Article