Isolation and characterization of Dichelobacter nodosus from ovine and caprine footrot in Kashmir, India.
Res Vet Sci
; 83(2): 141-4, 2007 Oct.
Article
em En
| MEDLINE
| ID: mdl-17250860
ABSTRACT
Footrot is a highly contagious and economically important disease of sheep and goats, caused by Dichelobacter nodosus, a slow growing anaerobic Gram-negative rod. The current Australian antigenic classification system, based on variation in the fimbriae, classifies D. nodosus into at least 10 serogroups (A-I and M) and 18 serotypes. This investigation was intended to determine the serological diversity of D. nodosus in this region of Kashmir, India. Exudates of footrot lesions were collected from 24 naturally infected sheep and 42 goats located in the Kashmir valley. Of these 66 samples, 24 yielded evidence of D. nodosus by PCR using 16SrDNA specific primers. Multiplex PCR using serogroup specific primers revealed the presence of serogroup B in all the samples except two, which showed the presence of serogroup E D. nodosus. This study also documents the isolation of D. nodosus and detection of serogroup E for the first time in India.
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Base de dados:
MEDLINE
Assunto principal:
Doenças dos Ovinos
/
Doenças das Cabras
/
Infecções por Bactérias Gram-Negativas
/
Dichelobacter nodosus
/
Pododermatite Necrótica dos Ovinos
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
Res Vet Sci
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Índia