Characterisation of the N-linked oligosaccharides of the light chain of human glycoprotein IIb by f.a.b.-m.s.
Carbohydr Res
; 221: 169-77, 1991 Dec 16.
Article
em En
| MEDLINE
| ID: mdl-1726195
The glycosylation of the light chain (GPIIbL) of glycoprotein IIb, one of the glycoproteins constituting the receptor for fibrinogen, fibronectin, and the von Willebrand factor on platelet cell surfaces, was investigated using fast-atom-bombardment mass spectrometry (f.a.b.-m.s.). Complex-type N-glycans were observed, attached to Asn-60. The most abundant oligosaccharide is a disialylated biantennary structure substituted with fucose on the chitobiose core. Mono-sialylated biantennary, and di- and tri-sialylated triantennary structures were found as minor constituents of the N-glycan population. The amino acid sequence of GPIIbL was fully mapped by f.a.b.-m.s., thereby providing the first direct evidence for the absence of O-glycosylation.
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Base de dados:
MEDLINE
Assunto principal:
Oligossacarídeos
/
Receptores Imunológicos
/
Glicoproteínas da Membrana de Plaquetas
/
Integrinas
/
Receptores de Superfície Celular
Limite:
Humans
Idioma:
En
Revista:
Carbohydr Res
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Reino Unido