RpoS as an intermediate in RsmA-dependent regulation of secondary antifungal metabolites biosynthesis in Pseudomonas sp. M18.
FEMS Microbiol Lett
; 268(1): 81-7, 2007 Mar.
Article
em En
| MEDLINE
| ID: mdl-17263850
ABSTRACT
To investigate the regulatory mechanism governing antifungal metabolite biosynthesis, two kinds of global regulator genes in Pseudomonas sp. M18, an rpoS and an rsmA gene, were cloned and mutated by inserting with an aacC1 cassette, respectively. Two mutants showed the same regulatory mode with repression of phenazine-1-carboxylic acid and remarkable enhancement of pyoluteorin. In the rpoS-mutant, a translational rsmA'-'lacZ fusion was expressed at the same level corresponding to that in the wild-type strain. In the rsmA-mutant, however, expression of the translational rpoS'-'lacZ fusion was only about 30% of that in the wild-type strain. The results indicated that the absence of RsmA leads to repression of the rpoS gene expression, which has further been confirmed with construction of chromosomal rpoS-lacZ fusion in the rsmA-mutant and the wild-type strain, respectively. The findings showed a new regulatory cascade controlling antifungal metabolite production in Pseudomonas sp. M18, suggesting that RpoS may serve as a mediator in RsmA-dependent regulation of secondary metabolite biosynthesis.
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Base de dados:
MEDLINE
Assunto principal:
Pseudomonas
/
Fator sigma
/
Proteínas de Bactérias
/
Regulação Bacteriana da Expressão Gênica
/
Antifúngicos
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
China