Optimization of protein expression systems for modern drug discovery.
Expert Rev Proteomics
; 4(1): 67-78, 2007 Feb.
Article
em En
| MEDLINE
| ID: mdl-17288516
ABSTRACT
The expression of high levels of stable and functional proteins remains a bottleneck in many scientific endeavors, including the determination of structures in a high-throughput fashion or the screening for novel active compounds in modern drug discovery. Recently, numerous developments have been made to improve the production of soluble and active proteins in heterologous expression systems. These include modifications to the expression constructs, the introduction of new and/or improved pro- and eukaryotic expression systems, and the development of improved cell-free protein synthesis systems. The introduction of robotics has enabled a massive parallelization of expression experiments, thereby vastly increasing the throughput and, hopefully, the output of such experiments. In addition, the big challenges of recombinant overexpression of membrane and secreted proteins are tackled, and some new methods are reviewed.
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Base de dados:
MEDLINE
Assunto principal:
Biossíntese de Proteínas
/
Clonagem Molecular
/
Avaliação Pré-Clínica de Medicamentos
Idioma:
En
Revista:
Expert Rev Proteomics
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
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