Identification of the C. elegans anaphase promoting complex subunit Cdc26 by phenotypic profiling and functional rescue in yeast.
BMC Dev Biol
; 7: 19, 2007 Mar 20.
Article
em En
| MEDLINE
| ID: mdl-17374146
ABSTRACT
BACKGROUND:
RNA interference coupled with videorecording of C. elegans embryos is a powerful method for identifying genes involved in cell division processes. Here we present a functional analysis of the gene B0511.9, previously identified as a candidate cell polarity gene in an RNAi videorecording screen of chromosome I embryonic lethal genes.RESULTS:
Whereas weak RNAi inhibition of B0511.9 causes embryonic cell polarity defects, strong inhibition causes embryos to arrest in metaphase of meiosis I. The range of defects induced by RNAi of B0511.9 is strikingly similar to those displayed by mutants of anaphase-promoting complex/cyclosome (APC/C) components. Although similarity searches did not reveal any obvious homologue of B0511.9 in the non-redundant protein database, we found that the N-terminus shares a conserved sequence pattern with the N-terminus of the small budding yeast APC/C subunit Cdc26 and its orthologues from a variety of other organisms. Furthermore, we show that B0511.9 robustly complements the temperature-sensitive growth defect of a yeast cdc26Delta mutant.CONCLUSION:
These data demonstrate that B0511.9 encodes the C. elegans APC/C subunit CDC-26.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Polaridade Celular
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Caenorhabditis elegans
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Genes cdc
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Meiose
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
BMC Dev Biol
Assunto da revista:
EMBRIOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article