Assessment of DNA damage at the dimer level: measurement of the formamide lesion.
Radiat Res
; 167(2): 146-51, 2007 Feb.
Article
em En
| MEDLINE
| ID: mdl-17390722
ABSTRACT
UVC-radiation-induced DNA damage was measured in mouse fibroblast cells using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in conjunction with isotopically labeled internal standards. The thymine glycol and formamide lesions were assayed in the form of modified dinucleoside monophosphates. The 8-oxo-7,8-dihydroguanine lesion was measured as the modified nucleoside. DNA damage in cells treated with tirapazamine was also measured. Tirapazamine is a chemotherapeutic agent that acts via a free radical mechanism. The two agents, UVC radiation and tirapazamine, produce markedly different profiles of DNA damage, reflecting their respective mechanisms of action. Both agents produce significant amounts of thymine glycol and formamide damage, but only the former produced a measurable amount of the 8-oxo-7,8-dihydroguanine lesion. The merits of measuring DNA damage at the dimer level are discussed.
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Base de dados:
MEDLINE
Assunto principal:
Dano ao DNA
/
DNA
/
Dimerização
/
Formamidas
Limite:
Animals
Idioma:
En
Revista:
Radiat Res
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos