Intraocular biodistribution of mono-L-aspartyl chlorin e6 in a primate choroidal neovascularization model.
Ophthalmic Surg Lasers Imaging
; 38(2): 142-7, 2007.
Article
em En
| MEDLINE
| ID: mdl-17396695
ABSTRACT
BACKGROUND AND OBJECTIVE:
To describe the biodistribution of a hydrophilic sensitizer, mono-L-aspartyl chlorin e6 (NPe6), in a primate model of choroidal neovascularization (CNV). MATERIALS ANDMETHODS:
NPe6 (25 mg/kg) and indocyanine green (ICG, 15 mg/kg) were intravenously administered simultaneously in monkeys with laser-induced CNVs. Eyes were enucleated and examined by fluorescence microscopy immediately, 20 minutes, and 1, 4, and 24 hours after dye injection.RESULTS:
Fluorescence peaked in retinal vessels immediately after dye administration; rapid washout began 1 hour postinjection. In contrast, experimental CNV demonstrated little fluorescence immediately after injection, with increased intensity at later time points. Peak dye fluorescence in CNV occurred 1 hour following dye injection. NPe6 fluorescence diminished rapidly and washed out completely by 24 hours. The dye accumulation and retention pattern of ICG dye resembled that of NPe6.CONCLUSIONS:
This study demonstrated the selective accumulation of NPe6 photosensitizer and ICG dye within experimental CNV and rapid clearance from the circulation and retinochoroidal tissue.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Porfirinas
/
Fármacos Fotossensibilizantes
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Neovascularização de Coroide
Limite:
Animals
Idioma:
En
Revista:
Ophthalmic Surg Lasers Imaging
Assunto da revista:
OFTALMOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Japão