Your browser doesn't support javascript.
loading
Clinical correlates of JAK2V617F allele burden in essential thrombocythemia.
Kittur, Jaya; Knudson, Ryan A; Lasho, Terra L; Finke, Christy M; Gangat, Naseema; Wolanskyj, Alexandra P; Li, Chin-Yang; Wu, Wenting; Ketterling, Rhett P; Pardanani, Animesh; Tefferi, Ayalew.
Afiliação
  • Kittur J; Division of Hematology, Department of Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA.
Cancer ; 109(11): 2279-84, 2007 Jun 01.
Article em En | MEDLINE | ID: mdl-17440984
BACKGROUND: JAK2V617F occurs in approximately 50% of patients with essential thrombocythemia (ET). Qualitative studies of mutation analysis have previously reported an association between JAK2V617F and advanced age, higher hemoglobin level, higher leukocyte count, and lower platelet count. A possible association with thrombotic complication has also been considered. METHODS: Allele-specific, quantitative polymerase chain reaction (PCR) analysis for JAK2V617F was performed in 176 patients with ET using genomic DNA from archived bone marrow, which was collected within 1 year (n=72 patients), between 1 and 5 years (n=64 patients), or after 5 years (n=40 patients) of diagnosis. RESULTS: JAK2V617F was detected in 96 patients (55%), in whom mutant allele burden ranged from 1% to 100% (median, 6.3%). Neither mutational frequency (P=.37) nor mutant allele burden (P=.62) was affected by the timing of bone marrow sample collection. The presence of JAK2V617F was found to be significantly associated with higher hemoglobin level (P<.0001), lower platelet count (P=.001), higher leukocyte count (P=.008), increased incidence of venous thrombosis occurring after diagnosis (P=.02), and older age at diagnosis (P=.03). All but age retained significance in multivariable analysis. In mutation-positive patients (n=96 patients), JAK2V617F allele burden clustered between 1% and 22% in 94 cases, in whom it correlated directly and significantly with platelet and leukocyte counts, palpable splenomegaly at diagnosis, and venous thrombosis occurring after diagnosis. The latter 2 associations remained significant with the inclusion of the remaining 2 outlier cases with 100% mutant allele burden; in addition, an association with male gender became evident. CONCLUSIONS: JAK2V617F allele burden imparts additional phenotypic effects in ET.
Assuntos
Buscar no Google
Base de dados: MEDLINE Assunto principal: Alelos / Janus Quinase 2 / Trombocitemia Essencial Tipo de estudo: Diagnostic_studies / Qualitative_research Limite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Cancer Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Estados Unidos
Buscar no Google
Base de dados: MEDLINE Assunto principal: Alelos / Janus Quinase 2 / Trombocitemia Essencial Tipo de estudo: Diagnostic_studies / Qualitative_research Limite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Cancer Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Estados Unidos