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Removal of estrogenic activity of 4-tert-octylphenol by ligninolytic enzymes from white rot fungi.
Tamagawa, Yuuki; Hirai, Hirofumi; Kawai, Shingo; Nishida, Tomoaki.
Afiliação
  • Tamagawa Y; Department of Environment and Forest Resources Science, Faculty of Agriculture, Shizuoka University, Ohya 836, Surugaku, Shizuoka, Japan.
Environ Toxicol ; 22(3): 281-6, 2007 Jun.
Article em En | MEDLINE | ID: mdl-17497634
ABSTRACT
4-tert-Octylphenol (4-t-OP) was treated with the white rot fungus Phanerochaete sordida YK-624 under ligninolytic condition with low-nitrogen and high-carbon culture medium. 4-t-OP completely disappeared after 5 days of treatment and the activities of ligninolytic enzymes, laccase and manganese peroxidase (MnP), were detected during this period, thus suggesting that the disappearance of 4-t-OP is related to these extracellular enzymes. Therefore, 4-t-OP was treated with laccase and MnP prepared from white rot fungi cultures. HPLC analysis demonstrated that 4-t-OP completely disappeared in the reaction mixture after 1 h of treatment with either laccase or MnP. Using the yeast two-hybrid assay system, it was also confirmed that laccase and MnP substantially removed the estrogenic activity of 4-t-OP after 1 and 2 h of treatment, respectively. These results strongly demonstrate that ligninolytic enzymes are effective in removing the estrogenic activity of 4-t-OP.
Assuntos
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Base de dados: MEDLINE Assunto principal: Peroxidases / Fenóis / Phanerochaete / Lacase / Estrogênios não Esteroides Idioma: En Revista: Environ Toxicol Assunto da revista: SAUDE AMBIENTAL / TOXICOLOGIA Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Japão
Buscar no Google
Base de dados: MEDLINE Assunto principal: Peroxidases / Fenóis / Phanerochaete / Lacase / Estrogênios não Esteroides Idioma: En Revista: Environ Toxicol Assunto da revista: SAUDE AMBIENTAL / TOXICOLOGIA Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Japão