Evaluation of a new method for identification of Cryptococcus neoformans which uses serologic tests aided by selected biological tests.
J Clin Microbiol
; 29(12): 2873-6, 1991 Dec.
Article
em En
| MEDLINE
| ID: mdl-1757563
ABSTRACT
A new method for identifying Cryptococcus neoformans isolates and their serotypes by the slide agglutination test using five kinds of factor sera, with the aid of nitrate reduction, phenol oxidase, and growth at 37 degrees C tests was evaluated by using 36 reference strains and 75 clinical isolates of C. neoformans. The results showed that the reference strains were identified exactly as they were labeled, and clinical isolates were identified as C. neoformans serotypes A, D, and AD. C. neoformans could be distinguished from other Cryptococcus species that cross-reacted with factor sera by their ability to grow at 37 degrees C. These results indicate that the slide agglutination test combined the use of factor sera for isolates which grow at 37 degrees C is a useful method for identification of C. neoformans and their serotypes and that the nitrate reduction test (negative in 100% of the isolates) and the phenol oxidase test (positive in approximately 95% of the isolates) can be used to confirm that the species is C. neoformans.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Tipagem Micológica
/
Cryptococcus neoformans
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Japão