Suppression of HIV replication using RNA interference against HIV-1 integrase.
FEBS Lett
; 581(17): 3253-9, 2007 Jul 10.
Article
em En
| MEDLINE
| ID: mdl-17592732
ABSTRACT
RNA interference (RNAi) has become one of the most powerful and popular approach on gene silencing in clinical research study especially in virology due to the gene-specific suppression property of small interfering RNA (siRNA). In this report, we demonstrate that expression of vector-mediated small hairpin RNA (shRNA) against human immunodeficiency virus type 1 (HIV-1) integrase (IN), one of the three important enzymes in HIV infection by controlling the integration of viral RNA to host DNA, could suppress the protein synthesis of EGFP-tagged IN in HeLa cell model efficiently. Furthermore, we show that IN shRNA can successfully reduce the HIV particles production in 293T cells at the level similar to the positive control of HIV-1 tat shRNA. These results provide the therapeutic possibility of HIV replication using RNAi against HIV-1 integrase.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Replicação Viral
/
HIV-1
/
Integrase de HIV
/
RNA Interferente Pequeno
/
Interferência de RNA
Tipo de estudo:
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
China