Artificial control of nitrate respiration through the lac promoter permits the assessment of oxygen-mediated posttranslational regulation of the nar operon in Pseudomonas aeruginosa.
J Bacteriol
; 189(17): 6501-5, 2007 Sep.
Article
em En
| MEDLINE
| ID: mdl-17616601
ABSTRACT
In this study, oxygen and nitrate regulation of transcription and subsequent protein expression of the unique narK1K2GHJI respiratory operon of Pseudomonas aeruginosa were investigated. Under the control of PLAC, P. aeruginosa was able to transcribe nar and subsequently express methyl viologen-linked nitrate reductase activity under aerobic conditions without nitrate. Modulation of PLAC through the LacI repressor enabled us to assess both transcriptional and posttranslational regulation by oxygen during physiological whole-cell nitrate reduction.
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Base de dados:
MEDLINE
Assunto principal:
Oxigênio
/
Pseudomonas aeruginosa
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Regulação Bacteriana da Expressão Gênica
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Nitrato Redutase
/
Nitratos
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos