[Study on the drug-resistant genes associated with beta-lactams and aminoglycosides in clinically isolated Pseudomonas aeruginosa].
Zhonghua Shao Shang Za Zhi
; 23(2): 108-11, 2007 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-17649884
ABSTRACT
OBJECTIVE:
To investigate drug-resistant genes associated with beta-lactams and aminoglycosides in clinically isolated Pseudomonas aeruginosa.METHODS:
Twenty strains of Pseudomonas aeruginosa were isolated from wound excretion of hospitalized burn patients. The strains resistant to 14 antibiotics were selected for detection of 16 kind of drug-resistant genes (TEM, SHV, OXA-10 cluster, PER, VEB, GES, CARB, CTX-M- I, IMP, VIM, SPM, GIM, DHA, MOX, FOX, oprD2) and 6 kind of aminoglycoside modification genes (aac(3)- I, aac(3)-II, aac(6')-I, aac(6')-II, ant (3")- I , ant(2")- I) in them by PCR.RESULTS:
Among the 20 strains resistant to beta-lactam , all of them were TEM and GES positive (100%), oprD2 gene depletion in 5 strains (25%). All other genes were negative. Among aminoglycoside resistant genes, 20 strains were aac (6') - I positive (100%), 7 were ant (2") - I positive (35%), and negative for other stains.CONCLUSION:
There were very high existence rates of TEM, GES and aac (6')- I genes in Pseudomonas aeruginosa isolated from clinical burn patients. The fact that GES-5 gene has also been detected in Pseudomonas aeruginosa, suggesting this organism is highly drug resistant in our burn unit.
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Base de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
/
Resistência beta-Lactâmica
/
Farmacorresistência Bacteriana Múltipla
/
Beta-Lactamas
/
Aminoglicosídeos
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
Zh
Revista:
Zhonghua Shao Shang Za Zhi
Assunto da revista:
TRAUMATOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article