Repression of kit expression by Plzf in germ cells.
Mol Cell Biol
; 27(19): 6770-81, 2007 Oct.
Article
em En
| MEDLINE
| ID: mdl-17664282
ABSTRACT
Male mice lacking expression of Plzf, a DNA sequence-specific transcriptional repressor, show progressive germ cell depletion due to exhaustion of the spermatogonial stem cell population. This is likely due to the deregulated expression of genes controlling the switch between spermatogonial self-renewal and differentiation. Here we show that Plzf directly represses the transcription of kit, a hallmark of spermatogonial differentiation. Plzf represses both endogenous kit expression and expression of a reporter gene under the control of the kit promoter region. A discrete sequence of the kit promoter, required for Plzf-mediated kit transcriptional repression, is bound by Plzf both in vivo and in vitro. A 3-bp mutation in this Plzf binding site abolishes the responsiveness of the kit promoter to Plzf repression. A significant increase in kit expression is also found in the undifferentiated spermatogonia isolated from Plzf(-/-) mice. Thus, we suggest that one mechanism by which Plzf maintains the pool of spermatogonial stem cells is through a direct repression of kit expression.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espermatogônias
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Regulação da Expressão Gênica
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Proteínas Proto-Oncogênicas c-kit
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Fatores de Transcrição Kruppel-Like
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Células Germinativas
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Itália