Stress fluctuations and motion of cytoskeletal-bound markers.
Phys Rev E Stat Nonlin Soft Matter Phys
; 76(1 Pt 1): 011918, 2007 Jul.
Article
em En
| MEDLINE
| ID: mdl-17677505
ABSTRACT
Cytoskeletal (CSK) dynamics such as remodeling and reorganization can be studied by tracking the spontaneous motion of CSK-bound particles. Particle motion is thought to be driven by local, ATP-dependent intracellular force fluctuations due to polymerization processes and motor proteins, and to be impeded by a viscoelastic, metastable cytoskeletal network. The mechanisms that link particle motion to force fluctuations and the CSK dynamics remain unclear. We report simultaneous measurements of the spontaneous motion of CSK-bound particles and of cellular force fluctuations. Cellular force fluctuations were measured by tracking fluorescent markers embedded in an elastic polyacrylamide hydrogel substrate that served as an extracellular matrix (ECM). The motion of CSK-bound particles and markers embedded in the ECM showed both persistence and superdiffusive behavior. Moreover, the movements of CSK-bound beads were temporally and spatially correlated with force fluctuations in the ECM. The findings suggest that the spontaneous motion of CSK-bound beads is driven not by random, local stress fluctuations within a viscoelastic continuum or cage, but rather by stress fluctuations within a tensed and constantly remodeling CSK network that transmits stresses over considerable distances to the ECM.
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Base de dados:
MEDLINE
Assunto principal:
Citoesqueleto
/
Movimento Celular
/
Proteínas da Matriz Extracelular
/
Proteínas do Citoesqueleto
/
Mecanotransdução Celular
/
Matriz Extracelular
/
Modelos Biológicos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Phys Rev E Stat Nonlin Soft Matter Phys
Assunto da revista:
BIOFISICA
/
FISIOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Alemanha