p53 expression and apoptosis in the lens after ultraviolet radiation exposure.
Invest Ophthalmol Vis Sci
; 48(9): 4187-91, 2007 Sep.
Article
em En
| MEDLINE
| ID: mdl-17724205
ABSTRACT
PURPOSE:
To localize p53 protein and active caspase-3 in the albino rat lens and to compare p53 mRNA and active caspase-3 expression in ultraviolet radiation (UVR) 300 nm exposed lenses and their contralateral nonexposed controls.METHODS:
Ten Sprague-Dawley albino rats were unilaterally exposed to 8 kJ/m(2) UVR, and the contralateral eyes were left nonexposed. In total, four exposed lenses and their respective contralateral nonexposed lenses were analyzed by immunohistochemistry to localize p53 and active caspase-3. In addition, six exposed and contralateral nonexposed lenses were analyzed by real-time RT-PCR. Quantified p53 and caspase-3 expression were compared between the in vivo UVR 300 nm exposed lenses and the contralateral nonexposed lenses.RESULTS:
All lenses exposed to UVR developed cataract. Immunohistochemistry showed that p53 and active caspase-3 were localized in the lens epithelial cells. Quantified p53 and caspase-3 expression were significantly higher in lenses exposed to UVR than in nonexposed lenses.CONCLUSIONS:
p53 and caspase-3 expression increase in lens epithelial cells after UVR exposure. In the lens, apoptosis induced by UVR may be associated with increased p53 expression.
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Base de dados:
MEDLINE
Assunto principal:
Lesões Experimentais por Radiação
/
Raios Ultravioleta
/
Catarata
/
Proteína Supressora de Tumor p53
/
Apoptose
/
Cristalino
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Invest Ophthalmol Vis Sci
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Suécia