Rab25 associates with alpha5beta1 integrin to promote invasive migration in 3D microenvironments.
Dev Cell
; 13(4): 496-510, 2007 Oct.
Article
em En
| MEDLINE
| ID: mdl-17925226
ABSTRACT
Here, we report a direct interaction between the beta1 integrin cytoplasmic tail and Rab25, a GTPase that has been linked to tumor aggressiveness and metastasis. Rab25 promotes a mode of migration on 3D matrices that is characterized by the extension of long pseudopodia, and the association of the GTPase with alpha5beta1 promotes localization of vesicles that deliver integrin to the plasma membrane at pseudopodial tips as well as the retention of a pool of cycling alpha5beta1 at the cell front. Furthermore, Rab25-driven tumor-cell invasion into a 3D extracellular matrix environment is strongly dependent on ligation of fibronectin by alpha5beta1 integrin and the capacity of Rab25 to interact with beta1 integrin. These data indicate that Rab25 contributes to tumor progression by directing the localization of integrin-recycling vesicles and thereby enhancing the ability of tumor cells to invade the extracellular matrix.
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Base de dados:
MEDLINE
Assunto principal:
Movimento Celular
/
Proteínas rab de Ligação ao GTP
/
Integrina alfa5beta1
/
Matriz Extracelular
/
Invasividade Neoplásica
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Dev Cell
Assunto da revista:
EMBRIOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Reino Unido