Pepitope: epitope mapping from affinity-selected peptides.
Bioinformatics
; 23(23): 3244-6, 2007 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-17977889
ABSTRACT
UNLABELLED Identifying the epitope to which an antibody binds is central for many immunological applications such as drug design and vaccine development. The Pepitope server is a web-based tool that aims at predicting discontinuous epitopes based on a set of peptides that were affinity-selected against a monoclonal antibody of interest. The server implements three different algorithms for epitope mapping PepSurf, Mapitope, and a combination of the two. The rationale behind these algorithms is that the set of peptides mimics the genuine epitope in terms of physicochemical properties and spatial organization. When the three-dimensional (3D) structure of the antigen is known, the information in these peptides can be used to computationally infer the corresponding epitope. A user-friendly web interface and a graphical tool that allows viewing the predicted epitopes were developed. Pepitope can also be applied for inferring other types of protein-protein interactions beyond the immunological context, and as a general tool for aligning linear sequences to a 3D structure. AVAILABILITY:
http//pepitope.tau.ac.il/
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Algoritmos
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Software
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Alinhamento de Sequência
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Mapeamento de Epitopos
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Análise de Sequência de Proteína
Idioma:
En
Revista:
Bioinformatics
Assunto da revista:
INFORMATICA MEDICA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Israel