The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation.
Biomaterials
; 29(6): 653-61, 2008 Feb.
Article
em En
| MEDLINE
| ID: mdl-17983651
ABSTRACT
Peripheral nerve regeneration can be enhanced by the stimulation of formation of bands of Büngner prior to implantation. Aligned electrospun poly(epsilon-caprolactone) (PCL) fibers were fabricated to test their potential to provide contact guidance to human Schwann cells. After 7 days of culture, cell cytoskeleton and nuclei were observed to align and elongate along the fiber axes, emulating the structure of bands of Büngner. Microarray analysis revealed a general down-regulation in expression of neurotrophin and neurotrophic receptors in aligned cells as compared to cells seeded on two-dimensional PCL film. Real-time-PCR analyses confirmed the up-regulation of early myelination marker, myelin-associated glycoprotein (MAG), and the down-regulation of NCAM-1, a marker of immature Schwann cells. Similar gene expression changes were also observed on cells cultured on randomly oriented PCL electrospun fibers. However, up-regulation of the myelin-specific gene, P0, was observed only on aligned electrospun fibers, suggesting the propensity of aligned fibers in promoting Schwann cell maturation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células de Schwann
Limite:
Humans
Idioma:
En
Revista:
Biomaterials
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos