Metacaspase 2 of Trypanosoma brucei is a calcium-dependent cysteine peptidase active without processing.
FEBS Lett
; 581(29): 5635-9, 2007 Dec 11.
Article
em En
| MEDLINE
| ID: mdl-18005666
ABSTRACT
Metacaspases are cysteine peptidases that are distantly related to the caspases, for which proteolytic processing is central to their activation. Here, we show that recombinant metacaspase 2 (MCA2) from Trypanosoma brucei has arginine/lysine-specific, Ca(2+)-dependent proteolytic activity. Autocatalytic processing of MCA2 occurred after Lys55 and Lys268; however, this was shown not to be required for the enzyme to be proteolytically active. The necessity of Ca(2+), but not processing, for MCA2 enzymatic activity clearly distinguishes MCA2 from the caspases and would be consistent with different physiological roles.
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Base de dados:
MEDLINE
Assunto principal:
Trypanosoma brucei brucei
/
Cisteína Endopeptidases
/
Proteínas de Protozoários
/
Cálcio
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Reino Unido