Investigation of DNA-protein sequence-specific interactions with a ds-DNA array.
Molecules
; 10(2): 417-26, 2005 Feb 28.
Article
em En
| MEDLINE
| ID: mdl-18007313
ABSTRACT
The sequence specific recognitions between DNAs and proteins play important roles in many biological functions. The use of double-stranded DNA arrays (ds-DNA arrays) for studying sequence specific recognition between DNAs and proteins is a promising method. Here we report the use of a ds-DNA probe with multi operation sites of restriction proteins in the middle sequence to investigate DNA-protein sequence-specific interactions including methylation. We arranged EcoR I site and Rsa I site on the same duplex DNA probe to fabricate ds-DNA arrays. We used the ds-DNA arrays to study DNA-restriction enzyme reactions before and after duplex DNA methylation under different probe concentration and reaction time conditions. Our results indicated that the ds-DNA arrays can be further biochemically modified and made accessible for interactions between DNAs and proteins in complex multi-step gene-regulation processes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Análise de Sequência de DNA
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Proteínas de Ligação a DNA
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
Molecules
Assunto da revista:
BIOLOGIA
Ano de publicação:
2005
Tipo de documento:
Article