Anti-prion activity generated by a novel vaccine formulation.
Neurosci Lett
; 429(2-3): 161-4, 2007 Dec 18.
Article
em En
| MEDLINE
| ID: mdl-18023980
ABSTRACT
Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) of domestic and wild cervids in North America. To address possible prevention regimens for CWD, we have used a mouse model system and the Rocky Mountain Laboratory (RML) mouse-adapted scrapie prion strain to screen efficacy of potential vaccine candidates. Three peptides derived from the primary amino acid sequence of the prion protein were conjugated to blue carrier protein (BCP) and formulated in an adjuvant containing M. avium subsp. avium. CL57/BL6 mice were vaccinated and boosted with 50 microg of the carrier protein-peptide conjugate formulation; all vaccines produced a humoral immune response as measured by ELISA. Disease challenge with the RML scrapie prion strain revealed anti-prion activity was generated by the vaccine formulations as measured by a delay in clinical disease onset and prolonged survivorship.
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Base de dados:
MEDLINE
Assunto principal:
Vacinas
/
Proteínas PrPSc
/
Doença de Emaciação Crônica
/
Imunoterapia
Limite:
Animals
Idioma:
En
Revista:
Neurosci Lett
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos