Multiparametric analysis of normal and postchemotherapy bone marrow: Implication for the detection of leukemia-associated immunophenotypes.
Cytometry B Clin Cytom
; 74(1): 17-24, 2008 Jan.
Article
em En
| MEDLINE
| ID: mdl-18061947
ABSTRACT
BACKGROUND:
The knowledge of normal marrow is mandatory to assess the malignant counterpart of normal cells and define leukemia-associated immunophenotypes (LAIPs). In this study, the expression of a variety of antigens expressed in normal and postchemotherapy bone marrow (BM) was analyzed to provide a frame of reference for the identification of myeloid LAIPs.METHODS:
Multiparameter four- and six-color flow cytometry was used to define antigen combinations totally absent or present at very minimal levels in marrow cells of normal individuals (n = 20) and patients receiving chemotherapy for acute lymphoblastic leukemia (n = 20). Immature (blast) cells were gated according to CD45/SSC properties. Fifty-three acute myeloid leukemia (AML) samples were studied in six-color combinations.RESULTS:
In six-color flow cytometry, 47 phenotypes were totally absent from blast gate in all normal samples. Forty-one other phenotypes were identified in less than 0.05% of blast cells. There was no difference between normal and postchemotherapy BMs. The four-color panel allowed to identify only 30 phenotypes present at a frequency <0.05%. Using the six-color panel, 58% of the absent or infrequent phenotypes in normal BM were found in at least one of 53 AML samples. All AML cases exhibited at least one LAIP.CONCLUSION:
Our results show that the ability to distinguish leukemic from healthy cells is considerably increased by a six-color approach. Furthermore, these absent or infrequent phenotypes in normal BM are identified in AML and can be utilized for minimal residual disease study.
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Base de dados:
MEDLINE
Assunto principal:
Medula Óssea
/
Leucemia Mieloide Aguda
/
Imunofenotipagem
/
Leucemia-Linfoma Linfoblástico de Células Precursoras
/
Citometria de Fluxo
/
Antineoplásicos
Tipo de estudo:
Diagnostic_studies
/
Risk_factors_studies
Limite:
Child
/
Humans
/
Middle aged
Idioma:
En
Revista:
Cytometry B Clin Cytom
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
França