Spike protein region (aa 636789) of porcine epidemic diarrhea virus is essential for induction of neutralizing antibodies.
Acta Virol
; 51(3): 149-56, 2007.
Article
em En
| MEDLINE
| ID: mdl-18076304
ABSTRACT
To analyze antigenic structure of the spike (S) protein of Porcine epidemic diarrhea virus (PEDV), the gene encoding its major immunodominant region S1 was amplified by PCR. We prepared four truncated S1 proteins spanning the entire S1 domain fused to GST protein. To identify the most important antigenic region of S1, the truncated S1-GST fusion proteins were examined for their ability to react with immune serum against PEDV and to elicit the formation of neutralization antibodies in immunized animals. We found that the region of S1 signed as S1D (aa 636789) was able to react with PEDV antiserum and to elicit formation of neutralization antibodies in mice. Moreover, the immune serum against S1D showed the binding ability to the native S protein of PEDV.
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Base de dados:
MEDLINE
Assunto principal:
Testes de Neutralização
/
Glicoproteínas de Membrana
/
Proteínas do Envelope Viral
/
Vírus da Diarreia Epidêmica Suína
/
Anticorpos Antivirais
/
Antígenos Virais
Limite:
Animals
Idioma:
En
Revista:
Acta Virol
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
China