Rapid detection and molecular serotyping of adenovirus by use of PCR followed by electrospray ionization mass spectrometry.
J Clin Microbiol
; 46(2): 644-51, 2008 Feb.
Article
em En
| MEDLINE
| ID: mdl-18094138
ABSTRACT
We have developed a PCR/electrospray ionization mass spectrometry (PCR/ESI-MS) assay for the rapid detection, identification, and serotyping of human adenoviruses. The assay employs a high-performance mass spectrometer to "weigh" the amplicons obtained from PCR using primers designed to amplify known human adenoviruses. Masses are converted to base compositions and, by comparison against a database of the genetic sequences, the serotype present in a sample is determined. The performance of the assay was demonstrated with quantified viral standards and environmental and human clinical samples collected from a military training facility. Over 500 samples per day can be analyzed with sensitivities greater than 100 genomes per reaction. This approach can be applied to many other families of infectious agents for rapid and sensitive analysis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Adenoviridae
/
Reação em Cadeia da Polimerase
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Infecções por Adenoviridae
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Espectrometria de Massas por Ionização por Electrospray
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Microbiologia Ambiental
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Guideline
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos