Identification of protein-protein interactions by mass spectrometry coupled techniques.
Adv Biochem Eng Biotechnol
; 110: 67-80, 2008.
Article
em En
| MEDLINE
| ID: mdl-18227982
ABSTRACT
The use of mass spectrometry in protein identification has revolutionized the field of proteomics. Coupled to various affinity purification techniques, mass spectrometry is used to identify protein-protein interactions. This chapter looks at the use of these affinity purification techniques in the identification of protein interactions. Various tags are used to purify protein complexes including tandem affinity purification. The FLAG tag is another commonly used tag which is a small tag that tends not to interfere with the protein function. These different affinity purification methods are used to purify proteins that are further identified by either ESI-MS or MALDI-MS.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
Proteínas
/
Mapeamento de Interação de Proteínas
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Adv Biochem Eng Biotechnol
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Canadá