Lamina-associated polypeptide 2alpha forms complexes with heat shock proteins Hsp70 and Hsc70 in vivo.
Biochem Biophys Res Commun
; 368(3): 767-71, 2008 Apr 11.
Article
em En
| MEDLINE
| ID: mdl-18261988
ABSTRACT
Lamina-associated polypeptide 2alpha (LAP2alpha), one of the alternatively spliced isoforms of the LAP2 gene, is a nucleoplasmic protein which forms oligomers and presumably associates to chromosomes via the LEM- and LEM-like regions. To characterize components of the LAP2alpha-containing complexes, we have expressed the alpha-specific C-terminal domain of LAP2alpha in HeLa cells and, after immunopurification, found that the heat shock proteins Hsp70 and Hsc70 reproducibly co-purified with this domain. Association between endogenous LAP2alpha and Hsp70 in non-transfected cells was confirmed by co-immunoprecipitation. The association was not mediated by the retinoblastoma protein.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Choque Térmico HSP70
/
Proteínas de Ligação a DNA
/
Proteínas de Choque Térmico HSC70
/
Proteínas de Membrana
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Áustria