UDP-glucosyltransferase activity toward exogenous substrates in Drosophila melanogaster.
Anal Biochem
; 194(2): 349-52, 1991 May 01.
Article
em En
| MEDLINE
| ID: mdl-1830726
To investigate the capacity of Drosophila extracts to glucosylate exogenous substrates we have developed a fast and sensitive method for the detection of UDP-glucosyltransferase activity using 4-nitrophenol, 1-naphthol, or 2-naphthol as substrates. High-performance liquid chromatography was used to separate and quantitate the reaction products, allowing detection of activities that produced as little as 1 pmol of 2-naphthol glucoside (fluorescence detection) or 16 pmol of 4-nitrophenol glucoside (absorbance detection). Optimal activity was found at 43 degrees C and alkaline pH. The affinity of the Drosophila enzyme was 250-fold higher for 1-naphthol or 2-naphthol (Km approximately 4 microM) than for 4-nitrophenol and UDP-glucose (Km approximately 1 mM).
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Base de dados:
MEDLINE
Assunto principal:
Difosfato de Uridina
/
Drosophila melanogaster
/
Glucosiltransferases
Limite:
Animals
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Espanha