Rapid typing of transmissible spongiform encephalopathy strains with differential ELISA.
Emerg Infect Dis
; 14(4): 608-16, 2008 Apr.
Article
em En
| MEDLINE
| ID: mdl-18394279
ABSTRACT
The bovine spongiform encephalopathy (BSE) agent has been transmitted to humans, leading to variant Creutzfeldt-Jakob disease. Sheep and goats can be experimentally infected by BSE and have been potentially exposed to natural BSE; however, whether BSE can be transmitted to small ruminants is not known. Based on the particular biochemical properties of the abnormal prion protein (PrPsc) associated with BSE, and particularly the increased degradation induced by proteinase K in the N terminal part of PrPsc, we have developed a rapid ELISA designed to distinguish BSE from other scrapie strains. This assay clearly discriminates experimental ovine BSE from other scrapie strains and was used to screen 260 transmissible spongiform encephalopathy (TSE)-infected small ruminant samples identified by the French active surveillance network (2002/2003). In this context, this test has helped to identify the first case of natural BSE in a goat and can be used to classify TSE isolates based on the proteinase K sensitivity of PrPsc.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Scrapie
/
Príons
/
Ensaio de Imunoadsorção Enzimática
/
Encefalopatia Espongiforme Bovina
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Emerg Infect Dis
Assunto da revista:
DOENCAS TRANSMISSIVEIS
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
França