Constitutive plasma membrane targeting and microdomain localization of Dok5 studied by single-molecule microscopy.
Biophys Chem
; 136(1): 13-8, 2008 Jul.
Article
em En
| MEDLINE
| ID: mdl-18455289
ABSTRACT
In the present study, single-molecule fluorescence microscopy was used to examine the characteristics of plasma membrane targeting and microdomain localization of enhanced yellow fluorescent protein (eYFP)-tagged wild-type Dok5 and its variants in living Chinese hamster ovary (CHO) cells. We found that Dok5 can target constitutively to the plasma membrane, and the PH domain is essential for this process. Furthermore, single-molecule trajectories analysis revealed that Dok5 can constitutively partition into microdomain on the plasma membrane. Finally, the potential mechanism of microdomain localization of Dok5 was discussed. This study provided insights into the characteristics of plasma membrane targeting and microdomain localization of Dok5 in living CHO cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Membrana Celular
/
Proteínas Adaptadoras de Transdução de Sinal
/
Microscopia
Limite:
Animals
Idioma:
En
Revista:
Biophys Chem
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
China